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bioRxiv · 10.64898/2026.05.15.725565

Autophagy flux during human aging is sex- and cell type-specific, and is associated with physical fitness

Abstract

Autophagy is widely proposed to decline with age; however, direct evidence for this across cell and tissue types in humans remains limited. Furthermore, it remains unknown whether interventions that improve physiological health during aging can modify autophagic activity in humans. Here, we performed transcriptomic and functional autophagy analyses across subject-matched human cell types from a healthy aging cohort spanning the adult lifespan. RNA-seq of primary dermal fibroblasts and induced neurons (iNs) revealed increased transcription of many autophagy-related genes with age, most markedly in fibroblasts. The impact of age on autophagic activity, measured using autophagy flux assays, was cell type- and sex-dependent, and uncoupled from autophagy-gene transcription. Autophagy flux decreased with age in male fibroblasts, was unchanged in female fibroblasts, and increased in female iNs. In freshly isolated peripheral blood mononuclear cells (PBMCs), autophagy flux became more heterogeneous with age and trended higher in older individuals, independent of sex. Although autophagy flux levels did not match across different cell types, higher autophagy flux in all cell types was associated with reduced physical function in older adults ([≥]70 years). Importantly, autophagy flux decreased following 12 weeks of mild exercise in parallel with improved physical function. These findings indicate that autophagy is regulated in a cell type-, sex-and physiological function-dependent manner during human aging, and highlight PBMC autophagy flux as a potentially modifiable, blood-accessible readout of physiological state in older adults.

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BibTeXRIS

Moreno, T. M., Heimler, S. R., Moran, R. J., Barkai, H. S., Scandalis, L., Traxler, L., Neil, A. R., Dozier, S., Bergstrom, J., Ranade, S. S., Bang, A. G., Mertens, J., Wing, D., Molina, A. J., Kumsta, C.. 2026-05-21. Autophagy flux during human aging is sex- and cell type-specific, and is associated with physical fitness. https://doi.org/10.64898/2026.05.15.725565

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