Functional consequences of centromeric satellite array asymmetry in female meiosis.

Ma, J.; Kumar, D.; Thaploo, A.; Ma, L.; Lampson, M. A.

doi:10.64898/2026.05.15.725525

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bioRxiv · 10.64898/2026.05.15.725525

Functional consequences of centromeric satellite array asymmetry in female meiosis.

Ma, J.·Kumar, D.·Thaploo, A.·Ma, L.·Lampson, M. A.

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Abstract

Centromeres are epigenetically specified chromosomal sites that support kinetochore assembly and often embedded within large satellite DNA arrays. Recent telomere to telomere genome assemblies have revealed extensive variation in centromeric satellite arrays between chromosomes and between individuals, but the functional significance of this variation remains unclear. To determine how satellite array size influences centromere function, we generated hybrid mouse models in which homologous chromosomes with different array sizes are paired in meiosis I, creating array size asymmetry across each meiotic bivalent. When an extremely small array is paired with a moderate size array, we find that array size asymmetry leads to functional asymmetry in both centromere chromatin and interactions with spindle microtubules, lagging chromosomes in anaphase I, and increased aneuploidy in MII eggs. In contrast, pairing an extremely large array with a moderate array does not lead to functional centromere asymmetry. Together, these results suggest a threshold model in which centromere array size is tolerated across a broad range, but minimal arrays become functionally limiting when paired with larger arrays in meiosis.

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Ma, J., Kumar, D., Thaploo, A., Ma, L., Lampson, M. A.. 2026-05-18. Functional consequences of centromeric satellite array asymmetry in female meiosis.. https://doi.org/10.64898/2026.05.15.725525

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