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bioRxiv · 10.64898/2026.05.12.724625

Wastewater and colloidal extracts of wastewater-irrigated soils select for resistant Acinetobacter baylyi beyond what measured antibiotic concentrations predict

Axtmann, K.·Paffenholz, C.·Auerhammer, A.·Michel-Farias, A.-K.·Heyde, B. J.·Coppers, L. M.·Braun, M.·Kappenberg, A.·Mulder, I.·Brueggen, S.·Siebe, C.·Amelung, W.·Siemens, J.·Bierbaum, G.
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Abstract

Numerous studies have shown that the abundance of antibiotic-resistant bacteria (ARBs) or antibiotic-resistance genes (ARGs) in soil increases after irrigation with wastewater. However, it is unclear whether this increase is due to the selection effects of pharmaceutical residues in the irrigation water or the continuous introduction of ARBs and ARGs with the wastewater. Further, it is unclear how the binding of antibiotics to natural colloids (1-1000 nm) affects their biological effects compared to truly dissolved substances (< 1 nm). We conducted competition experiments with resistant and susceptible Acinetobacter baylyi BD413 strains in wastewater, as well as in colloidal and truly dissolved extracts of soils irrigated with wastewater. Although the concentrations of our six target antibiotics were far below the measured minimum selective concentrations of the tested strains, we demonstrate that the resistant strain was favored in the wastewater and the colloidal extracts. In contrast, the truly dissolved fractions exhibited weaker and more variable selective effects. A non-targeted analysis revealed the presence of 82 additional substances in our extracts, including further antibiotics, pesticides, and different non-antibiotic drugs that may influence the selection of our resistant A. baylyi BD413 strain. Our findings suggest that antibiotic resistance is selected for in wastewater and wastewater-irrigated soils. This cannot be explained by antibiotic concentrations alone, but may also arise from the effects of complex mixtures of co-occurring contaminants, particularly those associated with colloidal particles.

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Axtmann, K., Paffenholz, C., Auerhammer, A., Michel-Farias, A.-K., Heyde, B. J., Coppers, L. M., Braun, M., Kappenberg, A., Mulder, I., Brueggen, S., Siebe, C., Amelung, W., Siemens, J., Bierbaum, G.. 2026-05-13. Wastewater and colloidal extracts of wastewater-irrigated soils select for resistant Acinetobacter baylyi beyond what measured antibiotic concentrations predict. https://doi.org/10.64898/2026.05.12.724625

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