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bioRxiv · 10.64898/2026.05.10.724069

Hamelia patens-Derived Red-Emitting Carbon Quantum Dots: Surface-State Luminescence, Antioxidant Potency, and In Vitro Bioimaging

Bhalerao, S.·Patil, J.·Mansuri, A. K.·Jain, S.·Kosara, S.·Prakash, G.·Kumar, D. A.·Bhatia, D. D.
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Abstract

Red-emitting carbon quantum dots (HP-CQDs) were synthesised for the first time from aqueous leaf extracts of Hamelia patens through single-step, reagent-free microwave-assisted carbonisation (750 W). The resulting nanoparticles displayed a narrow hydrodynamic size distribution centred at 3.9 nm, consistent with atomic force microscopy measurements showing a maximum height of 2.81 nm. Under 400 nm excitation, the CQDs exhibited a characteristic red emission maximum at 675 nm, representing a rare example of long-wavelength-emitting green CQDs derived from plant biomass. UV-Vis absorption bands at 224 and 256 nm were assigned to {pi}-{pi}* transitions of aromatic carbon domains and n-{pi}* transitions associated with carbonyl-containing surface groups, respectively. X-ray photoelectron spectroscopy (XPS) indicated a carbon-rich composition (C: 67.24%, O: 31.25%, N: 1.52%) with prominent C-O (42.67%) and C-C/C=C (42.64%) contributions. ATR-FTIR further confirmed the retention of hydroxyl, ether, and aliphatic functionalities following carbonisation. The excitation-wavelength-independent emission peak position implicates discrete surface molecular states rather than a heterogeneous distribution of emitters. HP-CQDs exhibit potent DPPH radical scavenging activity (ICOO = 141.8 {micro}g mLO{superscript 1}), comparable to ascorbic acid (ICOO = 114.8 {micro}g mLO{superscript 1}), and maintain >95% cell viability in both HeLa and RPE-1 cells up to 250 {micro}g mLO{superscript 1}. Confocal microscopy demonstrates concentration-dependent cytoplasmic accumulation and selective perinuclear localization at 300 {micro}g mLO{superscript 1}. In vivo biodistribution in zebrafish larvae confirms systemic uptake with statistically significant fluorescence enhancement at 500 {micro}g mLO{superscript 1} (p < 0.01), establishing HP-CQDs as biocompatible red-fluorescent probes with dual imaging-antioxidant functionality. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=148 SRC="FIGDIR/small/724069v2_ufig1.gif" ALT="Figure 1"> View larger version (61K): org.highwire.dtl.DTLVardef@17be668org.highwire.dtl.DTLVardef@153d874org.highwire.dtl.DTLVardef@1a7e6edorg.highwire.dtl.DTLVardef@29f13a_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Bhalerao, S., Patil, J., Mansuri, A. K., Jain, S., Kosara, S., Prakash, G., Kumar, D. A., Bhatia, D. D.. 2026-05-13. Hamelia patens-Derived Red-Emitting Carbon Quantum Dots: Surface-State Luminescence, Antioxidant Potency, and In Vitro Bioimaging. https://doi.org/10.64898/2026.05.10.724069

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