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bioRxiv · 10.64898/2026.05.06.722889

Inhibition in motion: Test-retest reliability of inhibitory kinematics in a go/no-go mouse tracking task

Mahesan, D.·Sharma, K.·Weinerth, M. K.·Dhaka, S.·Meinzer, M.·Fischer, R.
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Abstract

Response inhibition, the ability to suppress contextually inappropriate actions, is a cornerstone of cognitive control and is commonly assessed using paradigms such as the go/no-go task. However, traditional go/no-go paradigms rely on binary outcomes such as commission errors, which offer limited insight into the dynamic, graded behavioral adjustments underlying successful stopping. The present study developed a novel mouse-tracking go/no-go paradigm with a dynamic start to capture inhibitory processes during ongoing execution. Twenty-three healthy young adults completed the task in two sessions separated by approximately one week to evaluate the test-retest reliability of standard behavioral measures (error rates and reaction times), and three kinematic features: path length, mean velocity, and mean acceleration. Results revealed robust differences between go and no-go trials across all measures. Successful inhibition was characterized by significantly shorter path lengths and reduced mean velocity and acceleration compared to go trials. Critically, all measures demonstrated moderate-to-good test-retest reliability across sessions, with intraclass correlation coefficients ranging from .75 to .85 for go trials and from .59 to .83 for no-go trials. These findings establish construct validity and psychometric reliability of the current mouse-tracking go/no-go paradigm. The demonstrated stability of these measures provides the methodological foundation for their use in cross-sectional, longitudinal, and intervention research targeting inhibitory control.

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Mahesan, D., Sharma, K., Weinerth, M. K., Dhaka, S., Meinzer, M., Fischer, R.. 2026-05-09. Inhibition in motion: Test-retest reliability of inhibitory kinematics in a go/no-go mouse tracking task. https://doi.org/10.64898/2026.05.06.722889

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