Disruption of endothelial stability directly impacts vascular neighboring cells in Hereditary Hemorrhagic Telangiectasia

Climent, M.; Lambroia, L.; Sbalchiero, A.; Cassinelli, L.; Carriero, R.; Casali, C.; Cavallo, M.; Grizzi, F.; Pasqualini, F.; AAA Hegazi, M.; Introini, S.; Sirchia, F.; Olivieri, C.; Pagella, F.; Elia, L.

doi:10.64898/2026.05.05.723108

bioRxiv · 10.64898/2026.05.05.723108

Disruption of endothelial stability directly impacts vascular neighboring cells in Hereditary Hemorrhagic Telangiectasia

Climent, M.·Lambroia, L.·Sbalchiero, A.·Cassinelli, L.·Carriero, R.·Casali, C.·Cavallo, M.·Grizzi, F.·Pasqualini, F.·AAA Hegazi, M.·Introini, S.·Sirchia, F.·Olivieri, C.·Pagella, F.·Elia, L.

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Abstract

BACKGROUNDHereditary hemorrhagic telangiectasia (HHT) is a genetic disorder caused by pathogenic variants in the endothelial TGF{beta}/BMP pathway, crucial for the vascular arterial-venous differentiation. Vascular defects result in fragile and malformed vessels. The precise mechanisms driving vascular network failure remain incompletely understood, complicating the design of targeted therapies. METHODSNasal telangiectasias from HHT patients carrying variants in ACVRL1 or ENG were used to perform scRNA-seq (2 ACVRL1- and 1 ENG-patient) and spatial transcriptomics (1 ACVRL1 and 1 ENG) to uncover endothelial cells (EC) populations. Vascular characteristics within biopsies were evaluated using transmission electron microscopy (TEM) (1 ACVRL1 and 1 ENG) and histological analyses (23 ACVRL1 and 7 ENG), with particular attention to regions exhibiting varying degrees of damage. RESULTSComparing our HHT tissues with healthy donor from the literature, we identified cellular heterogeneity within EC populations, revealing two distinct venous clusters: a stable, quiescent population (Mature Vein) and an activated, pro-inflammatory population (HHT Vein). The coexistence of these two clusters suggests cellular diversity within the biopsy, further validated by TEM and histology, revealing a juxtaposition of well-organized collagen and cellular architecture with severely disrupted, fibrotic regions. Moreover, cellular crosstalk analyses allowed us to identify critical ligands in ECs that interact with fibroblasts and mural cells. In particular, we found Midkine (MDK) lost in HHT Vein ECs with further validation in vitro, suggesting its potential role in cellular stability. Furthermore, spatial transcriptomics allowed to further uncover pathologic phenotypes in cells neighboring HHT Vein ECs. CONCLUSIONSHHT biopsies exhibit localized inflamed and fibrotic vascular areas with the presence of different transcriptional sub-populations of EC. Within the same tissue, stable and activated ECs can be distinguished. The pathologic-like EC cluster, present exclusively in the HHT samples, may contribute to vascular leakage through the loss of important ligands involved in cellular communication.

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Climent, M., Lambroia, L., Sbalchiero, A., Cassinelli, L., Carriero, R., Casali, C., Cavallo, M., Grizzi, F., Pasqualini, F., AAA Hegazi, M., Introini, S., Sirchia, F., Olivieri, C., Pagella, F., Elia, L.. 2026-05-09. Disruption of endothelial stability directly impacts vascular neighboring cells in Hereditary Hemorrhagic Telangiectasia. https://doi.org/10.64898/2026.05.05.723108

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