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bioRxiv · 10.64898/2026.04.30.721989

Simple Electroporation of Chlamydomonas reinhardtii Strains with an Intact Cell Wall

Messmer, M.·de Carpentier, F.·Lam, E.·Hong, M.·Wakao, S.·Schroda, M.·Niyogi, K. K.
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Abstract

Chlamydomonas reinhardtii is a model green alga extensively used to study photosynthesis and cilia using molecular biology and genetics. Electroporation is a very common technique to transform DNA into the nuclear genome, which is essential to generate mutant collections and express transgenes. Here, we describe a simple, fast, and efficient protocol to transform strains with an intact cell wall. It achieves a good transformation efficiency without cell wall digestion or use of commercial kits and is compatible with the widely available Gene Pulser electroporation system. Key featuresO_LIHigh transformation efficiency of Chlamydomonas reinhardtii strains with an intact cell wall. C_LIO_LIFaster than currently available electroporation protocols. C_LI

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Messmer, M., de Carpentier, F., Lam, E., Hong, M., Wakao, S., Schroda, M., Niyogi, K. K.. 2026-05-05. Simple Electroporation of Chlamydomonas reinhardtii Strains with an Intact Cell Wall. https://doi.org/10.64898/2026.04.30.721989

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