Transvaginal ovum retrieval in scimitar horned oryx (Oryx dammah) and roan antelope (Hippotragus equinus)

Pennington, P. M.; Gillis, J. D.; Tourzani, D. A.; Lambert, C. J.; Nguyen, T. Q.; Metzler, S.; Citino, S. B.; James, M.; Penfold, L. M.; Herrick, J. R.

doi:10.64898/2026.04.30.721932

bioRxiv · 10.64898/2026.04.30.721932

Transvaginal ovum retrieval in scimitar horned oryx (Oryx dammah) and roan antelope (Hippotragus equinus)

Pennington, P. M.·Gillis, J. D.·Tourzani, D. A.·Lambert, C. J.·Nguyen, T. Q.·Metzler, S.·Citino, S. B.·James, M.·Penfold, L. M.·Herrick, J. R.

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Abstract

Development and use of assisted reproductive technologies (ARTs) in non-domestic species provides novel tools for species conservation. As a first step towards in vitro embryo production, we developed an OPU technique for two antelope species, scimitar horned oryx (Oryx dammah) and roan antelope (Hippotragus equinus) utilizing a custom-made needle guide and existing OPU equipment utilized by livestock and human practitioners. Females were anesthetized and placed in sternal recumbency for transvaginal OPUs. Prior to OPUs (36 - 45 hours), SHO and roan were either hormonally stimulated with follicle stimulating hormone (FSH, 140 or 250IU) as a single injection or not. A total of 32 and 26 OPUs were completed in SHO (n=10) and roan (n=7), respectively, representing one to four OPUs per animal at monthly intervals. A total of 141 oocytes were recovered from 215 follicles in SHO and 31 oocytes from 58 follicles in roan. FSH dose (250IU) increased (P<0.05) the number of follicles aspirated and the number of oocytes recovered in SHO. No effects of FSH were observed in roan (P>0.05). Good quality oocytes were recovered from all females and procedures were conducted in four consecutive months with no evidence of scar tissue buildup or reduced capacity to recover quality oocytes. These ARTs can be used to develop in vitro embryo production tools for population management and the preservation of female genetics; bolstering genetic diversity and guarding against extinction.

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Pennington, P. M., Gillis, J. D., Tourzani, D. A., Lambert, C. J., Nguyen, T. Q., Metzler, S., Citino, S. B., James, M., Penfold, L. M., Herrick, J. R.. 2026-05-05. Transvaginal ovum retrieval in scimitar horned oryx (Oryx dammah) and roan antelope (Hippotragus equinus). https://doi.org/10.64898/2026.04.30.721932

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