Development and Refinement of Microbial DNA Extraction Protocol from Bovine Milk

Cook, R.; Lima, J.; Dewhurst, R. J.; Huws, S. A.; Creevey, C. J.; Ferguson, H. J.

doi:10.64898/2026.04.30.721090

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bioRxiv · 10.64898/2026.04.30.721090

Development and Refinement of Microbial DNA Extraction Protocol from Bovine Milk

Cook, R.·Lima, J.·Dewhurst, R. J.·Huws, S. A.·Creevey, C. J.·Ferguson, H. J.

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Abstract

Milk is a challenging matrix to extract sufficient microbial DNA from for downstream analysis. This study assessed fourteen DNA extraction protocols for their DNA outputs. An adaptation of the QIAGEN DNeasy PowerSoil kit, which increased initial sample volume and maintained all volume of lysate following bead beating proved most effective.

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Cook, R., Lima, J., Dewhurst, R. J., Huws, S. A., Creevey, C. J., Ferguson, H. J.. 2026-05-01. Development and Refinement of Microbial DNA Extraction Protocol from Bovine Milk. https://doi.org/10.64898/2026.04.30.721090

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