Ectopic overproduction of cell wall glucan through membrane perturbation by an antifungal peptide theonellamide A in fission yeast

Nakao, K.; Carvalho, V. S. D.; Suganaga, A.; Osumi, M.; Tokukura, M.; Kakeya, H.; Matsuyama, A.; Yashiroda, Y.; Matsunaga, S.; Cortes, J. C. G.; Yoshida, M.; Ribas, J. C.; Nishimura, S.

doi:10.64898/2026.04.23.720496

bioRxiv · 10.64898/2026.04.23.720496

Ectopic overproduction of cell wall glucan through membrane perturbation by an antifungal peptide theonellamide A in fission yeast

Nakao, K.·Carvalho, V. S. D.·Suganaga, A.·Osumi, M.·Tokukura, M.·Kakeya, H.·Matsuyama, A.·Yashiroda, Y.·Matsunaga, S.·Cortes, J. C. G.·Yoshida, M.·Ribas, J. C.·Nishimura, S.

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Abstract

Ergosterol has multiple functions in filamentous fungi and yeasts, although only a part of the functions seems to be understood. An antifungal peptide, theonellamide A (TNM-A) induces drastic morphological changes in fission yeast cells by targeting plasma membrane ergosterol. TNM-A induces overproduction and ectopic accumulation of cell wall glucan at both growing tips and septum through a yet unknown mechanism. Here we show that TNM-A treatment causes accumulation of 1,3-{beta}-glucan at cell-polarity sites, not by increased activity of 1,3-{beta}-glucan synthase, but by an increased, persistent localization of the glucan synthase enzymes. Screening based on subcellular localization of proteins at periphery or polarity sites suggested the involvement of the Rho family GTPase Cdc42. In agreement, TNM-A induced both activation of Cdc42 and enhancement of membrane trafficking of glucan synthase enzymes. In conclusion, our chemical genetics analyses using TNM-A suggest that membrane ergosterol regulates the activity of Cdc42, which further regulates the localization of glucan synthases and cell wall biosynthesis. Highlights (four sentences)- Thenoellamide A (TNM-A) induces an ectopic overproduction of cell wall glucan. - TNM-A treatment causes increased, persistent localization of glucan synthases at the cell tips and septum. - TNM-A activates Cdc42 and upregulates membrane trafficking of glucan synthases. - Ergosterol is involved in proper activation/inactivation of Cdc42.

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Nakao, K., Carvalho, V. S. D., Suganaga, A., Osumi, M., Tokukura, M., Kakeya, H., Matsuyama, A., Yashiroda, Y., Matsunaga, S., Cortes, J. C. G., Yoshida, M., Ribas, J. C., Nishimura, S.. 2026-04-27. Ectopic overproduction of cell wall glucan through membrane perturbation by an antifungal peptide theonellamide A in fission yeast. https://doi.org/10.64898/2026.04.23.720496

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