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bioRxiv · 10.64898/2026.04.01.715762

muat: portable transformer-based method for tumour classification and representation learning from somatic variants

Sanjaya, P.·Pitkänen, E.
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Abstract

MotivationDeep neural networks have proven effective in classifying tumour types using next-generation sequencing data. However, developing transferable models that work across heterogeneous operating environments remains challenging due to differences in cohort compositions and data generation protocols, privacy concerns, and limited computational capabilities. ResultsWe introduce muat, a transformer-based software for tumour classification using somatic variant data from whole-genome (WGS) and whole-exome sequencing (WES). Building on previously developed MuAt and MuAt2 models, we distribute the software via Docker containers and Bioconda for deployment in high-performance computing (HPC) systems and Secure Processing Environments (SPEs). Using a downloadable MuAt checkpoint, we reproduce the performance reported in the original study on whole genome (PCAWG; 89% accuracy in histological tumour typing) and exome sequencing data (TCGA; 64% accuracy). Cross-cohort evaluation in Genomics England SPE achieved 81% accuracy without retraining and 89% following fine-tuning. As a demonstration of the softwares adaptability, we also deployed muat within the iCAN Digital Precision Cancer Medicine Flagships SPE and integrated it into a Nextflow-managed workflow. Availability and implementationmuat is available through conda (www.anaconda.org/bioconda/muat) and GitHub (https://github.com/primasanjaya/muat), under the Apache 2.0 License. Contactprima.sanjaya@helsinki.fi, esa.pitkanen@helsinki.fi; website: mlbiomed.net

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Sanjaya, P., Pitkänen, E.. 2026-04-03. muat: portable transformer-based method for tumour classification and representation learning from somatic variants. https://doi.org/10.64898/2026.04.01.715762

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