The nuclear transport receptor Impβ is a regulator of actin polymerization

Fahrenkrog, B.; Guo, H.; Mahmoud, A. H. H. H.; Neumann, P.; Armbruster, P.; Rencurel, C.; Newton, R.; Kapinos, L. E.; Dickmanns, A.; Lim, R. Y. H.

doi:10.64898/2026.03.30.715331

bioRxiv · 10.64898/2026.03.30.715331

The nuclear transport receptor Impβ is a regulator of actin polymerization

Fahrenkrog, B.·Guo, H.·Mahmoud, A. H. H. H.·Neumann, P.·Armbruster, P.·Rencurel, C.·Newton, R.·Kapinos, L. E.·Dickmanns, A.·Lim, R. Y. H.

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Abstract

Nuclear transport receptors are best known for mediating nucleocytoplasmic transport (NCT) through nuclear pore complexes. Here, we uncover an unexpected function of the primary import receptor importin-{beta} (Imp{beta}) as a direct regulator of the actin cytoskeleton. Imp{beta} associates with stress fibers and the cell cortex and promotes actin polymerization through direct interactions. Disrupting Imp{beta}-actin binding impairs stress fiber formation and suppresses cell migration well before NCT is affected. In 3D spheroids, perturbing Imp{beta} further compromises tissue integrity, as reflected in changes to nuclear curvature and ellipticity. Together, these findings identify Imp{beta} as a regulator of actin organization in both 2D and 3D contexts, revealing a direct link between the nuclear transport machinery and the cytoskeleton.

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Fahrenkrog, B., Guo, H., Mahmoud, A. H. H. H., Neumann, P., Armbruster, P., Rencurel, C., Newton, R., Kapinos, L. E., Dickmanns, A., Lim, R. Y. H.. 2026-03-31. The nuclear transport receptor Impβ is a regulator of actin polymerization. https://doi.org/10.64898/2026.03.30.715331

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