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bioRxiv · 10.64898/2026.03.30.715300

An atlas of transcriptional dynamics in maternal blood over the course of healthy pregnancy

Feenstra, B.·Hede, F. R. D.·Piening, B. D.·Skotte, L.·Nastou, K.·Liang, L.·Fadista, J.·Rasmussen, M.-L. H.·Scheller, N. M.·Jiang, C.·Vallania, F.·Wei, E.·Liu, Q.·Chaib, H.·Geller, F.·Boyd, H. A.·Snyder, M. P.·Melbye, M.
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Abstract

Pregnancy results in profound physiological changes driven by dynamic and precisely programmed molecular processes. Maternal peripheral blood is generally the specimen of choice for studying these processes, as it is easily accessible and essential for many aspects of maintaining a healthy pregnancy. Here, we present a high-resolution atlas of the dynamic temporal changes in the transcriptome of maternal peripheral blood in healthy human pregnancy. We generated comprehensive RNA sequencing data in 802 weekly samples from 31 healthy pregnant women from the first trimester until after delivery. Using a strict discovery and replication setup, our longitudinal analysis of gene expression identified 720 genes with robust pregnancy-specific expression patterns. Using weighted graph correlation network analysis, we identified nine pregnancy-associated transcriptional modules that reveal a strong, coordinated enrichment of innate/neutrophil and antiviral immune programs, alongside changes in adaptive immunity (T cell differentiation and signaling), erythropoiesis and hemoglobin metabolism. Cell-type deconvolution revealed that these transcriptomic shifts were accompanied by increased relative neutrophil proportions and reduced naive CD4 and CD8 T cells in pregnancy. We provide a comprehensive characterization of dynamic changes across pregnancy, highlighting maternal blood as a key systemic regulator in healthy gestation. Together, our findings establish a reference atlas of healthy pregnancy, which can be used to identify dysregulated processes and mechanisms in women with pregnancy complications. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=168 SRC="FIGDIR/small/715300v1_ufig1.gif" ALT="Figure 1"> View larger version (34K): org.highwire.dtl.DTLVardef@e6dd97org.highwire.dtl.DTLVardef@de217dorg.highwire.dtl.DTLVardef@168ad94org.highwire.dtl.DTLVardef@15c0dd1_HPS_FORMAT_FIGEXP M_FIG C_FIG O_LI720 genes showed robust pregnancy specific expression patterns. C_LIO_LICo-expression analysis clustered the genes into nine modules with distinct dynamics. C_LIO_LIEnrichment in pathways involved in innate and neutrophil-mediated immunity, antiviral responses, T cell differentiation and signaling, erythropoiesis and hemoglobin metabolism. C_LIO_LICell-type deconvolution showed increases in neutrophils and decreases in naive CD4 and CD8 T cells. C_LIO_LIThe atlas of detailed longitudinal transcriptional changes provides a baseline reference for healthy pregnancy. C_LIO_LIResults for all genes and protein-protein interaction networks are made available for interactive exploration. C_LI

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Feenstra, B., Hede, F. R. D., Piening, B. D., Skotte, L., Nastou, K., Liang, L., Fadista, J., Rasmussen, M.-L. H., Scheller, N. M., Jiang, C., Vallania, F., Wei, E., Liu, Q., Chaib, H., Geller, F., Boyd, H. A., Snyder, M. P., Melbye, M.. 2026-04-01. An atlas of transcriptional dynamics in maternal blood over the course of healthy pregnancy. https://doi.org/10.64898/2026.03.30.715300

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