Hierarchical Assembly of Native Cytoplasmic Lattices Revealed by Cryo-EM

Zhang, Y.-L.; Liu, Y.; Liu, Q.; Zhang, Q.; Jin, J.; Liu, S.; Yang, S.; Peng, Y.-J.; Lai, M.; Sun, Y.; Zhang, Y.-Y.; Mei, Y.; Tong, X.; Zhang, S.; Gui, M.

doi:10.64898/2026.03.30.715201

bioRxiv · 10.64898/2026.03.30.715201

Hierarchical Assembly of Native Cytoplasmic Lattices Revealed by Cryo-EM

Zhang, Y.-L.·Liu, Y.·Liu, Q.·Zhang, Q.·Jin, J.·Liu, S.·Yang, S.·Peng, Y.-J.·Lai, M.·Sun, Y.·Zhang, Y.-Y.·Mei, Y.·Tong, X.·Zhang, S.·Gui, M.

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Abstract

Oocytes depend on maternal factors for maturation and early embryogenesis. Cytoplasmic lattices (CPLs) are distinctive fibrillar structures in mammalian oocyte cytoplasm implicated in maternal factor storage and developmental competence, yet their molecular composition and architecture remain elusive. Here, we use cryo-electron microscopy to resolve the high-resolution structure of native mouse oocyte CPLs. We identify 14 intrinsic components of multiple copies that assemble into filaments in which PADI6-mediated self-assembly and two subtypes of subcortical maternal complexes (SCMCs) form the core scaffold. Unexpectedly, we discover that /{beta}-tubulin dimers and two autoinhibited ubiquitination modules (UHRF1-UBE2D3, SKP1-FBXW18) are internal components of CPLs. We also uncover multivalent interactions that organize adjacent CPL filaments into higher-order helical bundles. By integrating CPL structure with biochemical and proteomic data, we propose a hierarchical assembly mechanism of CPLs, from subcomplexes to filaments to supramolecular lattices. Our study offers mechanistic insights into how maternal proteins are spatially organized to ensure successful early embryonic development and provides a structural foundation for understanding female infertility related to CPL-associated gene mutations.

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Zhang, Y.-L., Liu, Y., Liu, Q., Zhang, Q., Jin, J., Liu, S., Yang, S., Peng, Y.-J., Lai, M., Sun, Y., Zhang, Y.-Y., Mei, Y., Tong, X., Zhang, S., Gui, M.. 2026-04-01. Hierarchical Assembly of Native Cytoplasmic Lattices Revealed by Cryo-EM. https://doi.org/10.64898/2026.03.30.715201

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