An integrated single cell and spatial omics atlas of human prenatal development

Webb, S.; Rose, A.; Xu, C.; Steele, L.; Kuri, M. A.; Stephenson, E.; Inecik, K.; Jafree, D.; Foster, A. R.; Basurto-Lozada, D.; Chipampe, N.-J.; Pournara, A. V.; Jacques, M.-A.; To, K.; Admane, C.; Kritikaki, E.; Chroscik, M. A.; Horsfall, D.; Foreman, J.; Rademaker, K.; Karjalainen, J.; Laddach, A.; Madad, S.; Lawrence, J. E. G.; Kleshchevnikov, V.; Lisgo, S.; Lee, J. T. H.; Blevinal, J.; Alqahtani, A.; Makarchuk, S.; Jackson, J.; Ucuncu, E.; Silva, T. P.; Lorenzi, V.; Torabi, F.; Botting, R. A.; Roberts, K.; Olabi, B.; Chakala, K. P.; Dony, L.; Dall'Aglio, G.; Cujba, A.-M.; Whitfield, H. J.

doi:10.64898/2026.03.30.714220

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bioRxiv · 10.64898/2026.03.30.714220

An integrated single cell and spatial omics atlas of human prenatal development

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Abstract

Single cell genomics has enabled analysis of human prenatal development at unprecedented resolution. However, most studies have relied on dissociated tissues during restricted windows of development, limiting insights into how spatially distributed networks of cells, and multicellular niches emerge and adapt to distinct organ microenvironments in situ. Moreover, existing human developmental atlases have not yet been harmonised, and we thus lack a comprehensive catalogue of known cell types in the developing human body. Here, we introduce the Human Developmental Cell Atlas (HDCA), a unified structural, cellular and molecular resource for prenatal human development. The HDCA integrates published and unpublished single cell/nucleus RNAseq atlases across prenatal organs, and includes a newly generated, spatially resolved, multimodal cell atlas of intact human embryos. Spanning 4-22 post conceptional weeks, capturing embryonic and early to mid fetal stages, the HDCA contains [~]4.6 million cells/nuclei which resolve into [~]450 cell types, explorable with a bespoke web portal. For a global overview of the human embryos multicellular communities, we applied unsupervised deep learning to our intact human embryo spatial data, charting 114 tissue niches that are structural and signalling hubs for the cellular interactions of the embryo. Guided by these niches, we profiled cellular networks over space and time, not examinable using single-organ atlases. In so doing, we revealed tissue-specific fibroblast patterning from previously undescribed mesenchyme progenitors, early diversification of organ-specific blood capillaries and lymphatic vasculature, emergence of neural crest cell fates, the formation of placode-and neural crest-derived peripheral sensory neurons, and how tissue niches guide peripheral neuron maturation and axonal migration. The HDCA thus serves as a comprehensive step towards a comprehensive understanding of human prenatal development, and a template towards unravelling the biology of congenital disorders.

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Webb, S., Rose, A., Xu, C., Steele, L., Kuri, M. A., Stephenson, E., Inecik, K., Jafree, D., Foster, A. R., Basurto-Lozada, D., Chipampe, N.-J., Pournara, A. V., Jacques, M.-A., To, K., Admane, C., Kritikaki, E., Chroscik, M. A., Horsfall, D., Foreman, J., Rademaker, K., Karjalainen, J., Laddach, A., Madad, S., Lawrence, J. E. G., Kleshchevnikov, V., Lisgo, S., Lee, J. T. H., Blevinal, J., Alqahtani, A., Makarchuk, S., Jackson, J., Ucuncu, E., Silva, T. P., Lorenzi, V., Torabi, F., Botting, R. A., Roberts, K., Olabi, B., Chakala, K. P., Dony, L., Dall'Aglio, G., Cujba, A.-M., Whitfield, H. J.. 2026-04-01. An integrated single cell and spatial omics atlas of human prenatal development. https://doi.org/10.64898/2026.03.30.714220

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