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bioRxiv · 10.64898/2026.03.24.712950

Deficits in tail-lift and air-righting reflexes in rats after ototoxicity associate with loss of vestibular type I hair cells

Palou, A.·Tagliabue, M.·Beraneck, M.·Llorens, J.
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Abstract

The rat vestibular system plays a critical role in anti-gravity responses such as the tail-lift reflex and the air-righting reflex. In a previous study in male rats, we obtained evidence that these two reflexes depend on the function of non-identical populations of vestibular sensory hair cells (HC). Here, we caused graded lesions in the vestibular system of female rats by exposing the animals to several different doses of an ototoxic chemical, 3,3-iminodipropionitrile (IDPN). After exposure, we assessed the anti-gravity responses of the rats and then assessed the loss of type I HC (HCI) and type II HC (HCII) in the central and peripheral regions of the crista, utricle and saccule. As expected, we recorded a dose-dependent loss of vestibular function and loss of HCs. The relationship between hair cell loss and functional loss was examined using non-linear models fitted by orthogonal distance regression. The results indicated that both the tail-lift reflex and the air-righting reflexes mostly depend on HCI function. However, a different dependency was found on the epithelium triggering the reflex: while the tail-lift response is sensitive to loss of crista and/or utricle HCIs, the air-righting response rather depends on utricular and/or saccular integrity.

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Palou, A., Tagliabue, M., Beraneck, M., Llorens, J.. 2026-03-26. Deficits in tail-lift and air-righting reflexes in rats after ototoxicity associate with loss of vestibular type I hair cells. https://doi.org/10.64898/2026.03.24.712950

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