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bioRxiv · 10.64898/2026.03.23.713132

VESTIBULAR FUNCTION LOSS ASSOCIATES WITH SENSORY EPITHELIUM PATHOLOGY IN VESTIBULAR SCHWANNOMA PATIENTS

Borrajo, M.·Callejo, A.·CASTELLANOS, E.·Amilibia, E.·Llorens, J.
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Abstract

Vestibular schwannomas (VS) cause vestibular function loss by mechanisms still poorly understood. We evaluated the vestibulo-ocular reflex by the video-assisted Head Impulse Test (vHIT) in patients with planned tumour resection by a trans-labyrinthine approach. The vestibular sensory epithelia were collected and processed by immunofluorescent labelling for confocal microscopy analysis of sensory hair cell subtypes (type I, HCI, and type II, HCII), calyx endings of the pure-calyx afferents, and the calyceal junction normally found between HCI and the calyx (n=23). Comparing Normo-vHIT and Hypo-vHIT patients, we concluded that worse vestibular function associates with decreased HCI and HCII counts in the sensory epithelia and with increased proportion of damaged calyces. A decrease in the number of HCI and calyx endings of the pure-calyx afferents was recorded to associate with age increase. Partial least squares regression (PLSR) models indicated that VS and age had independent, additive effects on vestibular function. Correlation analyses indicated that lower vHIT gains associate with lower numbers of HCI and increased percentages of damaged calyces. These data support the hypothesis that the deleterious effect of VS on vestibular function is mediated, at least in part, by its damaging impact on the vestibular sensory epithelium. They also provide further evidence for the dependency of the vestibulo-ocular reflex on HCI function and for the calyceal junction pathology as a common response of the sensory epithelium to HC stress.

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Borrajo, M., Callejo, A., CASTELLANOS, E., Amilibia, E., Llorens, J.. 2026-03-25. VESTIBULAR FUNCTION LOSS ASSOCIATES WITH SENSORY EPITHELIUM PATHOLOGY IN VESTIBULAR SCHWANNOMA PATIENTS. https://doi.org/10.64898/2026.03.23.713132

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