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bioRxiv · 10.64898/2026.03.19.712668

Serotonergic axons signal reward, sensory stimulation, and prepare for movement in primary somatosensory cortex

Przibylla, P.·Buetfering, C.·von Engelhardt, J.
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Abstract

Serotonin is one of the main neuromodulators in the brain, involved in regulating mood, complex behaviors and sensory input. Serotonin reaches primary somatosensory cortex (S1) via axons of neurons located in the dorsal raphe nucleus (DRN). DRN neurons can be modulated, amongst others, by reward, sensory stimulation, or movement but the activity pattern of serotonergic neurons targeting S1 is not known. Therefore, it is unclear under which circumstances serotonin is released in S1. Here, we expressed GCaMP8 in serotonergic neurons of the DRN to analyze the activity of their axons in S1 using two-photon Ca2+-imaging. Cluster analysis of axonal activities suggests that one to four functional groups of serotonergic axon segments project to a 0.3 mm2 horizontal plane of S1. We show that activity in serotonergic axons is strongly driven by reward and weakly by sensory stimulation of the whiskers. Movement, however, is preceded by a modulation, up and down, of the serotonergic signal seconds before the running onset. In summary, rewards and sensory stimulation lead to activity in serotonergic axons which is likely to adjust signal processing in S1 upon these events. The serotonergic signal changes seconds before movement onset probably preparing the neural network in S1 for the state change that accompanies running.

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Przibylla, P., Buetfering, C., von Engelhardt, J.. 2026-03-20. Serotonergic axons signal reward, sensory stimulation, and prepare for movement in primary somatosensory cortex. https://doi.org/10.64898/2026.03.19.712668

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