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bioRxiv · 10.64898/2026.03.16.711072

Htr3a receptors control attenuation of fear responses by modulating the corticolimbic network activity and synchronization

Zewdie, S.·MARISSAL, T.·Birot, G.·Schaller, K.·Dayer, A.·Quairiaux, C.
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Abstract

The fear circuit orchestrates defensive responses to environmental threats and is essential for survival. Dysregulation of this system is thought to contribute to the pathophysiology of several psychiatric disorders. Within this fear circuit, the corticolimbic network, particularly the amygdala and the medial prefrontal cortex (mPFC), is strongly modulated by serotonin. Previous studies have shown that Htr3a knockout (Htr3a-KO) mice exhibit deficits in the extinction of cued fear memory; however, the circuit level mechanisms underlying these impairments remain unknown. Here, we investigated this question by recording local field potentials evoked by auditory conditioned stimuli (CS) in the prelimbic (PrL), infralimbic (IL), and basolateral amygdala (BLA) of head-fixed wild-type (WT) and Htr3a-KO mice prior to fear conditioning and during fear memory retrieval. Behaviorally, Htr3a-KO mice displayed a delayed attenuation of fear-induced freezing during cued fear memory retrieval, whereas WT mice showed a rapid attenuation in freezing. Electrophysiologically, Htr3a-KO mice exhibited reduced fear-evoked theta power in the PrL, IL, and BLA, along with diminished mPFC-BLA theta synchrony. Moreover, theta-phase modulation of gamma oscillations within the BLA, which has been shown to increase during fear states, was perturbed in the absence of Htr3a signaling. Together, these findings indicate that Htr3a is critical for maintaining proper oscillatory dynamics within the mPFC-BLA circuit and for supporting effective attenuation of learned fear. Highlights- Attenuation of fear responses during fear memory retrieval sessions is protracted in Htr3a knock-out mice - The fear-induced theta response in the medial prefrontal cortex and the basolateral amygdala is less powerful in the Htr3a knock-out mice than in wild-type - Htr3a knock-out mice show a deficit in fear-induced synchronization as well as in theta modulation of gamma power in the cortico-limbic network - These results suggest that malfunction of the Htr3a receptor cause alterations in fear network circuit mechanisms that might be linked to deficits in fear responses attenuation

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Zewdie, S., MARISSAL, T., Birot, G., Schaller, K., Dayer, A., Quairiaux, C.. 2026-03-18. Htr3a receptors control attenuation of fear responses by modulating the corticolimbic network activity and synchronization. https://doi.org/10.64898/2026.03.16.711072

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