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bioRxiv · 10.64898/2026.03.12.711374

Orthogonal cell division organizes surface virulence factors to drive staphylococcal abscess community formation

Abstract

During infection, Staphylococcus aureus forms dense multicellular structures, called staphylococcal abscess communities (SACs), that are encased in a capsule made of host fibrin to evade host immune defenses. S. aureus cells divide characteristically along successive orthogonal planes, but the contribution of this division geometry to infection is unclear. Here, we show that disrupting orthogonal cell division by deleting the cell division septum placement factor PcdA impairs SAC formation in vivo and in a three-dimensional in vitro model. Loss of PcdA leads to uneven surface distribution of adhesins containing the YSIRK signal sequence that directs their insertion into the division septum, thereby resulting in uneven interaction with fibrin fibers. Consequently, bacterial communities fail to establish a robust fibrin pseudocapsule and remain accessible to immune cells. We propose that orthogonal cell division coordinates cell cycle progression with extracellular matrix engagement, SAC architecture, and persistence within host tissues. HIGHLIGHTSO_LIOrthogonal cell division promotes staphylococcal abscess community formation C_LIO_LILoss of PcdA disrupts fibrin pseudocapsule assembly in 3D models C_LIO_LIDivision geometry ensures uniform surface deployment of adhesins C_LIO_LICell division plane selection links bacterial cell cycle control to virulence C_LI

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BibTeXRIS

Ramos-Leon, F., Altouma, V., Goldberg, P., Ibrahim, A., D'Atri, D., Machinandiarena, F., Verdi, V., Missiakas, D. M., Davis, K. M., Ramamurthi, K. S.. 2026-03-12. Orthogonal cell division organizes surface virulence factors to drive staphylococcal abscess community formation. https://doi.org/10.64898/2026.03.12.711374

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