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bioRxiv · 10.64898/2026.03.06.710172

Architecture of the Gβγ-prefusion SNARE complex reveals the molecular mechanism of inhibition of vesicle fusion

Eitel, A. R.·Young, M.·Cassada, J.·Bell, E. W.·Meiler, J.·Hamm, H. E.
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Abstract

Presynaptic inhibitory GPCR (Gi/o GPCR) signaling is an essential regulatory mechanism in vertebrate physiology. Near the presynaptic active zone, Gi/o GPCR activation releases G-protein {beta}{gamma} heterodimers (G{beta}{gamma}) which act to inhibit synaptic vesicle fusion through either modulation of Ca2+ entry via voltage-gated Ca2+ channels, or by direct interactions with the core exocytotic machinery comprised of the ternary SNARE complex downstream of Ca2+ influx. The precise molecular mechanism underlying G{beta}{gamma}-SNARE mediated inhibition has remained unclear due to lack of structural data for the G{beta}{gamma}-SNARE complex. We address this long-standing question here by stabilizing the interaction between G{beta}1{gamma}2 and a pre-fusion ternary SNARE mimetic and determining the structure using single-particle cryo-EM. We used our cryo-EM envelope to build an atomic level prediction of the interaction interface. We validated key interaction sites predicted by our model at the C-terminus of SNAP-25 using site directed mutagenesis and biochemical affinity measurements. Additionally, we found that G{beta}1{gamma}2 and a fragment of the regulatory protein complexin can engage the pre-fusion SNARE complex simultaneously. On the basis of these results, we propose a model in which G{beta}1{gamma}2 acts on the partially zipped SNARE complex at a late stage in the vesicle docking and priming cycle. In the model, the amino-terminal coiled-coil of G{beta}1{gamma}2 forms an interface with the C-terminus of the target membrane SNARE (t-SNARE) complex to prevent complete incorporation of the vesicle SNARE (v-SNARE) into the core SNARE helical bundle, thus blocking vesicle approach to the plasma membrane. The {beta}-propeller domain of G{beta}1 may also sterically hinder vesicle approach. Together these results provide crucial structural insights into the mechanism of binding of G{beta}{gamma} to the SNARE complex, and lends essential insights into the critical role of GPCR signaling to the SNARE complex in modulating synaptic vesicle fusion.

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Eitel, A. R., Young, M., Cassada, J., Bell, E. W., Meiler, J., Hamm, H. E.. 2026-03-07. Architecture of the Gβγ-prefusion SNARE complex reveals the molecular mechanism of inhibition of vesicle fusion. https://doi.org/10.64898/2026.03.06.710172

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