Bioabsorbable Magnesium Metal ScaffoldsImprove Dermal Wound Healing and Tissue Regeneration

Guerra, M. E.; Anika, N. N.; Nagi, A.; Hopkins, T. M.; An, X.; Yu, L.; Liu, P.; Lee, C.; Keswani, S. G.; Avila, R.; Pixley, S. K.; Balaji, S.

doi:10.64898/2026.03.03.709352

bioRxiv · 10.64898/2026.03.03.709352

Bioabsorbable Magnesium Metal ScaffoldsImprove Dermal Wound Healing and Tissue Regeneration

Guerra, M. E.·Anika, N. N.·Nagi, A.·Hopkins, T. M.·An, X.·Yu, L.·Liu, P.·Lee, C.·Keswani, S. G.·Avila, R.·Pixley, S. K.·Balaji, S.

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Abstract

ObjectiveEvaluate the effects of bioabsorbable magnesium wires on dermal wound healing and tissue regeneration in a murine full-thickness wound model. Approach6 mm diameter stented dorsal skin wounds were created in C57BL/6J mice and treated with implanted WE43B magnesium alloy wires or PBS control. Wound healing was evaluated on days 7 and 28 by histology, immunohistochemistry, and micro-CT. Finite element analysis modeled mechanical strain distribution due to wire degradation during healing. ResultsAt day 7, magnesium wire-treated wounds showed 100% improved granulation tissue formation, reduced inflammation (37% fewer CD45+ leukocytes and 37% fewer F4/80+ macrophages), increased neovascularization (91% more CD31+ lumens), and 74% more nerve bundles. Improved wound closure (mean difference -1.48 mm) did not reach statistical significance (d = 1.06). By day 28, magnesium-treated wounds showed improved collagen organization and normalized epidermal thickness. The increase in dermal appendages (247%), and vascular density (41%) did not reach statistical significance. Micro-CT confirmed progressive wire degradation. Modeling revealed that degrading wires dynamically altered strain gradients in healing tissue, thereby modulating the spatial mechanical cues that govern fibroblast migration and extracellular matrix (ECM) remodeling. InnovationMagnesium is an essential trace element involved in cellular processes critical to wound repair, including angiogenesis, nerve growth, inflammation modulation, and ECM remodeling. Previous magnesium delivery systems incorporated polymers or other confounding materials that degrade rapidly. We directly applied bioabsorbable pure magnesium metal to provide sustained ion release and favorable mechanical properties to support regenerative healing. ConclusionBioabsorbable magnesium wires support regenerative wound healing by reducing inflammation, enhancing neovascularization, and promoting favorable ECM remodeling without adverse inflammatory reactions. These findings provide a strong rationale to harness magnesium metal use in wound healing applications.

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Guerra, M. E., Anika, N. N., Nagi, A., Hopkins, T. M., An, X., Yu, L., Liu, P., Lee, C., Keswani, S. G., Avila, R., Pixley, S. K., Balaji, S.. 2026-03-05. Bioabsorbable Magnesium Metal ScaffoldsImprove Dermal Wound Healing and Tissue Regeneration. https://doi.org/10.64898/2026.03.03.709352

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