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bioRxiv · 10.64898/2026.03.03.709284

Biological implications of a detailed repeat annotation in Octopus vulgaris

Bonar, M.·Elliot, T. A.·Ahmadi, M. A.·Cottenie, K.·Linquist, S.
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Abstract

Octopuses are phenotypically distinctive organisms, and recent genomic work raises questions about the contributions of transposable elements (TE) to their genomic architecture. We leveraged a robust repeat annotation pipeline, in combination with manual and automated curatorial techniques, to produce a more comprehensive repeat annotation of Octopus vulgaris. This revealed that [~]66% of the genome are repeats, in contrast to previous estimates of 43-50%. Whereas previous studies of TE expansion in Octopus bimaculoides identified two bursts of activity, 25 and 56 MYA, our re-annotation revealed four such expansions at 18, 25, 33, and 56 MYA. We further identified a landscape of TE hot- and cold spots. This much refined TE timescape and landscape will serve as a useful basis for understanding TE contributions to O. vulgaris evolution, and also for identifying factors contributing to variation in the TE community across genomic space and evolutionary time.

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Bonar, M., Elliot, T. A., Ahmadi, M. A., Cottenie, K., Linquist, S.. 2026-03-05. Biological implications of a detailed repeat annotation in Octopus vulgaris. https://doi.org/10.64898/2026.03.03.709284

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