Sex-specific Genetic Regulatory Effects in Chickens

Zhang, H.; Lin, B.; Xi, Y.; Zhu, D.; Peng, C.; Tu, J.; Liu, H.; GUAN, D.; Ouyang, Q.; Liu, B.; Fan, C.; Song, Z.; Meng, X.; Li, H.; Zheng, W.; Zhu, X.; An, B.; Li, Z.; Wang, Y.; Lu, J.; Wang, M.; Teng, J.; Lu, X.; Cui, L.; Li, B.; Guo, S.; Xie, K.; Li, Y.; Madsen, O.; Ionita-Laza, I.; Wright, D.; Godia, M.; Xiang, R.; Wang, X.; Hao, D.; Zhang, Q.; Yin, Y.; Yi, G.; Wang, Z.; Zhou, C.; Sun, C.; Hu, X.; Yang, N.; Darrington, C.; Hsu, S.-H.; Zhao, X.; Zhou, H.; van der Wijst, M.; Zeng, J.; Liu, L.; Shi, S.; Hou, Y.; Zhao, G.; Zhao, P.; Fang, L.; He, X.

doi:10.64898/2026.02.26.708179

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bioRxiv · 10.64898/2026.02.26.708179

Sex-specific Genetic Regulatory Effects in Chickens

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Abstract

Sexual dimorphism is a defining vertebrate feature, yet its sex-specific molecular architecture remains poorly understood. Here we established a sex-balanced, uniformly reared chicken cohort to map this landscape, integrating individual whole-genome sequencing with 7,969 bulk and 779,380 single nucleus transcriptomes across 32 tissues from 280 birds. We identified 495,098 independent expression quantitative trait loci for 20,194 genes, including 10,937 loci modulated by cell-type composition. Notably, 340 genes were regulated by 449 loci in a sex-dependent manner, significantly enrichment in endocrine tissues like adipose and the adrenal gland. Furthermore, we fine-mapped 1,219 structural variants, demonstrating their unique roles to tissue- and sex-specific expression beyond SNPs. Ultimately, we showed the utility of these regulatory effects in elucidating the molecular basis of metabolism and complex traits in both chickens and humans. This comprehensive atlas of regulatory effects provides profound insights into the genomic and molecular basis of sexual dimorphism in vertebrates.

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Zhang, H., Lin, B., Xi, Y., Zhu, D., Peng, C., Tu, J., Liu, H., GUAN, D., Ouyang, Q., Liu, B., Fan, C., Song, Z., Meng, X., Li, H., Zheng, W., Zhu, X., An, B., Li, Z., Wang, Y., Lu, J., Wang, M., Teng, J., Lu, X., Cui, L., Li, B., Guo, S., Xie, K., Li, Y., Madsen, O., Ionita-Laza, I., Wright, D., Godia, M., Xiang, R., Wang, X., Hao, D., Zhang, Q., Yin, Y., Yi, G., Wang, Z., Zhou, C., Sun, C., Hu, X., Yang, N., Darrington, C., Hsu, S.-H., Zhao, X., Zhou, H., van der Wijst, M., Zeng, J., Liu, L., Shi, S., Hou, Y., Zhao, G., Zhao, P., Fang, L., He, X.. 2026-02-28. Sex-specific Genetic Regulatory Effects in Chickens. https://doi.org/10.64898/2026.02.26.708179

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