Brain Kappa Opioid Receptor Availability Across Stress and Social Buffering Conditions: A Positron Emission Tomography Study in Coppery Titi Monkeys

Manca, C.; Paulus, J. P.; D Almeida, A. J.; Caceres, A.; Sosnowski, M. J.; Hobson, B. A.; Ferrer, E.; Chaudhari, A. J.; Bales, K. L.

doi:10.64898/2026.02.17.706461

bioRxiv · 10.64898/2026.02.17.706461

Brain Kappa Opioid Receptor Availability Across Stress and Social Buffering Conditions: A Positron Emission Tomography Study in Coppery Titi Monkeys

Manca, C.·Paulus, J. P.·D Almeida, A. J.·Caceres, A.·Sosnowski, M. J.·Hobson, B. A.·Ferrer, E.·Chaudhari, A. J.·Bales, K. L.

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Abstract

Social connectedness strongly influences health and longevity, and adult pair bonds provide psychological benefits distinct from other social relationships. Oxytocin (OT), corticotropin-releasing hormone (CRH), and opioids, play an important role in the formation and maintenance of pair bonds. Evidence suggests that OT modulates the stress response via the hypothalamic-pituitary-adrenal (HPA) axis, while the kappa ({kappa}) opioid system interacts with and may modulate OT signaling in contexts of stress and separation. In this study 20 coppery titi monkeys were exposed to a physical stressor under three social conditions: baseline (no stressor, partner present), stress (stressor, no partner present) and buffering (stressor, partner present). We predicted stress-induced dynorphin release would reduce {kappa}-opioid receptor availability measured via [{superscript 1}{superscript 1}C]GR103545 Positron Emission Tomography (PET) and lower cerebrospinal fluid (CSF) OT, whereas partner presence would mitigate dynorphin release and increase CSF OT, with reduced dynorphin inferred from higher {kappa}-opioid receptor radioligand binding. Our results show condition-dependent differences in [{superscript 1}{superscript 1}C]GR103545 binding in several brain regions, including the amygdala and hippocampus, with altered binding in both the stress and social buffering conditions. Cortisol levels were elevated in the stress condition compared to baseline. Females exhibited lower CSF OT levels during stress than at baseline, whereas plasma OT levels did not differ across conditions or between sexes. Spearman correlations revealed no significant associations between plasma and CSF OT. Together, these findings highlight the complex interaction between {kappa}-opioid signaling, OT, and HPA axis activity in the context of social relationships and highlight neuroendocrine mechanisms underlying stress regulation in pair-bonded species.

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Manca, C., Paulus, J. P., D Almeida, A. J., Caceres, A., Sosnowski, M. J., Hobson, B. A., Ferrer, E., Chaudhari, A. J., Bales, K. L.. 2026-02-18. Brain Kappa Opioid Receptor Availability Across Stress and Social Buffering Conditions: A Positron Emission Tomography Study in Coppery Titi Monkeys. https://doi.org/10.64898/2026.02.17.706461

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