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bioRxiv · 10.64898/2026.02.11.703394

The impact of dietary fibers on gut microbiome of young children: insights from ex vivo experiments and an observational cohort

Dogra, S. K.·Sprenger, N.·Wang, D.
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Abstract

During weaning period, the intake of dietary fibres changes and increases dramatically. Given the considerable structural differences, we hypothesized that different fibres may vary in their function. The objective of the study was to explore the impact of specific dietary fibres (arabinoxylan, cellulose, pectin and xyloglucan) on the gut microbiome of children below 3 years. By using ex vivo fecal fermentation experiments, cellular models and cohort data analysis, we assessed how these fibres and their combinations influence infants gut microbiota composition, diversity, metabolite production and possible actions on the gut epithelial barrier function. We found that the fermentation with arabinoxylan, xyloglucan and pectin resulted in an increased production of short-chain fatty acids. These fibres also promoted the generation of metabolites with potential health benefits, such as indole-3-lactic acid. By combining the ex vivo fermentation and cellular co-culture experiments, arabinoxylan and xyloglucan were found to be able to maintain gut epithelial barrier integrity upon lipopolysaccharide challenge, and a blend of cellulose, pectin, and xyloglucan dampened different LPS induced cytokines. Moreover, pectin was found supporting the growth of a wide range of microbial species ex vitro and correlated positively with -diversity in young children in an observational cohort. Our findings provided insights into the potential benefits of diverse fibre intakes during early life. Further studies are needed to understand the mechanisms and the effectiveness of specific fibres on the gut microbiome development in young children.

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Dogra, S. K., Sprenger, N., Wang, D.. 2026-02-11. The impact of dietary fibers on gut microbiome of young children: insights from ex vivo experiments and an observational cohort. https://doi.org/10.64898/2026.02.11.703394

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