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bioRxiv · 10.64898/2026.02.10.705075

Adaptive gene transcription in Escherichia coli under environmental stress

Ge, P.·Rashid, F.-Z. M.·Gaarthuis, L. K. F.·Cajili, M. K.·Tan, M.·Pang, B.·Schnetz, K.·Dame, R. T.
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Abstract

Escherichia coli is highly sensitive to acid and osmotic stress but adapts by modulating the expression of stress responsive genes. Nucleoid-associated proteins (NAPs) play key roles in DNA organization and sensing environmental changes. The histone-like nucleoid structuring protein H-NS is an NAP acting as a global regulator of stress genes. H-NS may alter local chromatin structure to modulate the expression of such genes in response to environmental stress. The H-NS homolog StpA co-regulates several target genes, but its precise role is poorly defined. To investigate the regulatory interplay between these two proteins, we examined transcription, DNA binding and chromatin structure at two regulated operons, hdeAB and proVWX, in E. coli following exposure to acid and salt shock. Our results show that H-NS senses pH and osmotic cues to remodel chromatin and relieve repression, while StpA compensates for H-NS loss, particularly at proVWX, highlighting a coordinated regulatory network.

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Ge, P., Rashid, F.-Z. M., Gaarthuis, L. K. F., Cajili, M. K., Tan, M., Pang, B., Schnetz, K., Dame, R. T.. 2026-02-11. Adaptive gene transcription in Escherichia coli under environmental stress. https://doi.org/10.64898/2026.02.10.705075

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