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bioRxiv · 10.64898/2026.02.04.703799

MEA-LINK identifies the CCL4-CCR5 axis in neuronal hyperactivity control by human microglia

Mordelt, A.·Scheefhals, N.·Schuurmans, I. M. E.·Slottje, K.·Rivera, M. C.·Hommersom, M. P.·Huang, A.·Bichmann, L.·Lewerissa, E. I.·van Hugte, E. J. H.·Schubert, D.·Tsang, J. S.·Kasri, N. N.·de Witte, L. D.
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Abstract

Microglia, the resident immune cells of the brain, act along a spectrum to maintain CNS homeostasis, respond to perturbations, and control neuronal activity. Disentangling the molecular mechanisms of human microglia-neuron crosstalk remains challenging due to the context-dependent, dynamic nature of their interaction. We introduce MEA-LINK, a systems-approach leveraging natural variation to screen for immune modulators of neuronal activity. This multi-modal platform integrates human induced pluripotent stem cell (hiPSC) technology with micro-electrode array (MEA) recordings and proteomic analyses of secreted immune factors, allowing for longitudinal samples and correlations across modalities. We applied MEA-LINK to explore microglia-neuron interactions during development and hyperactivity challenges. We show that human microglia accelerate neuronal network development and rescue hyperactive network phenotypes. Linking the secretome adaptations to neuronal network activity variations, we identified CCL4 as a top candidate in microglia-mediated hyperactivity control. Then, we functionally validated the context-dependent role of microglial CCL4 to neuronal CCR5 signaling in human neuronal networks. Our findings support a neuron-specific function of chemokines and their receptors in the brain and provide a new perspective for immune signaling in neuronal hyperactivity control. The MEA-LINK platform thus offers a foundation for comprehensive, systematic studies of human microglia-neuron interactions. HighlightsO_LIMEA-LINK integrates micro-electrode array recordings with proteomics of longitudinal samples to identify immune modulators of neuronal activity. C_LIO_LIHuman microglia rescue neuronal hyperactivity induced by pharmacological and genetic challenges. C_LIO_LIMicroglial CCL4 dampens neuronal activity via CCR5 signaling in a context-dependent manner. C_LI Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=147 SRC="FIGDIR/small/703799v1_ufig1.gif" ALT="Figure 1"> View larger version (39K): org.highwire.dtl.DTLVardef@18e44f7org.highwire.dtl.DTLVardef@151c450org.highwire.dtl.DTLVardef@12f7b89org.highwire.dtl.DTLVardef@57651b_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Mordelt, A., Scheefhals, N., Schuurmans, I. M. E., Slottje, K., Rivera, M. C., Hommersom, M. P., Huang, A., Bichmann, L., Lewerissa, E. I., van Hugte, E. J. H., Schubert, D., Tsang, J. S., Kasri, N. N., de Witte, L. D.. 2026-02-06. MEA-LINK identifies the CCL4-CCR5 axis in neuronal hyperactivity control by human microglia. https://doi.org/10.64898/2026.02.04.703799

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