RNAseK is vital for epithelial proton pump activity in Drosophila melanogaster

Abstract

The transporting epithelial tissues comprising the Drosophila melanogaster alimentary and renal systems are known to share a common set of enriched genes sometimes referred to as the "epitheliome", reflecting their shared transport functions. Core amongst these genes are the vha genes, which encode subunits of the large Vacuolar-type ATPase (V-ATPase) proton pump complex. However, many of the non-vha components of the epitheliome remain broadly uncharacterised. Here, we explore the role of RNAseK, a gene identified during unbiased epithelial screens in Drosophila whose function within insects is not yet known, though evidence from mammalian systems suggest a role in supporting proton pump activity. We demonstrate computationally that RNAseK is strongly conserved across evolutionary history, and that expression is regulated by the highly epithelial-specific dCLEAR motif. Seeking to understand why epithelial expression is so emphasised, we have assayed the effects of RNAseK knockdown in different epithelia throughout the fly. Across hindgut, midgut, and Malpighian tubules, we note profound defects in gross tissue morphology, transport activity, and fly survival. Mechanistically, our findings that RNAseK may co-localise with the V-ATPase complex, and that V-ATPase inhibition phenocopies RNAseK knockdown, suggest that RNAseK is a critical component of the proton transport axis across Drosophila tissues. Summary statementRNAseK is enriched throughout, and required in, Drosophila melanogaster epithelial tissues. Molecular evidence and evolutionary inferences suggest this is due to a role in the proton transport axis.