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bioRxiv · 10.64898/2026.02.02.703378

Sequential restriction of SNARE-mediated fusion by the COPII inner coat and SNARE chaperones

Mackey, E. J.·Takenaka, T.·Miller, E. A.·Merz, A. J.
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Abstract

SNARE-mediated fusion requires assembly of four SNARE domains (R, Qa, Qb, and Qc) distributed across two membranes, with an SM (Sec1/Munc-18 family) chaperone catalyzing this assembly. We investigated topological requirements for the four SNAREs that mediate ER-Golgi fusion using in vitro assays. In the presence of the cognate SM Sly1, we find that a single topology drives efficient fusion: the R SNARE on one membrane versus Qa/Qb/Qc SNAREs on the other. These results prompted us to look upstream, at COPII-SNARE interactions. The assembled COPII coat is known to block fusion. We discovered that of five COPII core subunits, the Sar1 GTPase and Sec23/Sec24 subunits were necessary and sufficient to prevent fusion for prolonged periods, and that Sar1 was dispensable for inhibition over short periods. When Sec24- interactions with vesicle SNAREs were disrupted, inhibition was relieved, indicating that SNARE sequestration by COPII prevents fusion. These observations help to explain how appropriate fusion events are facilitated while inappropriate events are deterred.

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Mackey, E. J., Takenaka, T., Miller, E. A., Merz, A. J.. 2026-02-04. Sequential restriction of SNARE-mediated fusion by the COPII inner coat and SNARE chaperones. https://doi.org/10.64898/2026.02.02.703378

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