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bioRxiv · 10.64898/2026.02.01.703181

Sex-dependent involvement of lateral septum astrocytes in social fear: Role of oxytocin receptor signaling

Boi, L.·Menon, R.·Denis, C.·Wang, K.-Y.·Petitjean, H.·Di Benedetto, B.·Charlet, A.·Neumann, I.
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Abstract

Astrocytes are now widely recognized as important modulators of synaptic plasticity and socio-emotional behaviors. Recent studies highlight their involvement in anxiety- and depressive-like behaviors, particularly via oxytocin (OXT) signaling. While the specific contributions of astrocytes remain largely unexplored, the role of OXT receptor (OXTR) signaling in the lateral septum (LS) in regulating social fear expression has been well characterized. Here, we studied the differential contribution of astrocytic OXTR signaling using a social fear conditioning (SFC+) paradigm. We found the highest abundance of astrocytes, and especially of OXTR-expressing (OXTR+) astrocytes, within the caudal LC (LSc) compared to the rostral LS in both male and female mice. Interestingly, female mice displayed a significantly higher number of astrocytes and OXTR+ astrocytes in the LSc in comparison to males. However, social fear acquisition resulted in dynamic changes in LSc astrocytic morphology and calcium activity in male mice. Furthermore, we showed that pre-SFC acquisition pharmacology-induced loss of local astrocytic function facilitated the extinction of social fear in males. In support, astrocyte-specific OXTR knockdown in the LSc also facilitated social fear extinction in both males and females. Taken together, our study identifies OXTR-signaling in LSc astrocytes as a crucial component in the mechanisms underlying the regulation of social fear in a sex-dependent manner.

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Boi, L., Menon, R., Denis, C., Wang, K.-Y., Petitjean, H., Di Benedetto, B., Charlet, A., Neumann, I.. 2026-02-02. Sex-dependent involvement of lateral septum astrocytes in social fear: Role of oxytocin receptor signaling. https://doi.org/10.64898/2026.02.01.703181

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