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bioRxiv · 10.64898/2026.01.30.702603

The glycine-arginine-rich motif of 53BP1 modulates RNA interactions necessary for its liquid-liquid phase separation during DNA Damage Response

Terraneo, F.·Ceccon, M.·Sapkota, O.·Zheng, T.·Brizioli, M.·dello Stritto, A.·Cummings, S.·Codispoti, S.·Ossolengo, G.·Orsenigo, F.·Magni, S.·Gottinger, A.·Mattevi, A.·Fuxreiter, M.·Fawzi, N. L.·Lottersberger, F.·Giavazzi, F.·d'Adda di Fagagna, F.
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Abstract

The DNA damage response relies on the rapid assembly of repair factors into foci with properties of liquid-liquid phase separation, driven by de novo transcription of damage-induced RNAs. 53BP1 is a key component of these condensates, yet the molecular determinants driving this process remain unknown. Here, through computational, structural and in vitro approaches, we identify the oligomerization domain of 53BP1 and its glycine-arginine-rich (GAR) motif as crucial for RNA interactions and phase separation. Biophysical characterization reveals that 53BP1-RNA condensates can progressively mature into a more stable state, and that GAR mutants display aberrant material properties. Using a cellular model of telomere fusion events, we demonstrate that the GAR motif is essential for 53BP1-mediated DNA repair, which depends on the combined contributions of RNA binding and appropriate condensate biophysical properties. Therefore, RNA-driven 53BP1 condensation is functionally required to maintain genome integrity.

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Terraneo, F., Ceccon, M., Sapkota, O., Zheng, T., Brizioli, M., dello Stritto, A., Cummings, S., Codispoti, S., Ossolengo, G., Orsenigo, F., Magni, S., Gottinger, A., Mattevi, A., Fuxreiter, M., Fawzi, N. L., Lottersberger, F., Giavazzi, F., d'Adda di Fagagna, F.. 2026-01-30. The glycine-arginine-rich motif of 53BP1 modulates RNA interactions necessary for its liquid-liquid phase separation during DNA Damage Response. https://doi.org/10.64898/2026.01.30.702603

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