Chondroitin 4-sulphate depletion enhances synaptic plasticity and memory in aging

Svobodova Burianova, J.; Svoboda, J.; Ruzicka, J.; Stepankova, K.; Klausova, T.; Gmiterkova, L.; Spundova, T.; Martinez-Varea, N.; Kralikova, M.; Turecek, R.; Machova Urdzikova, L.; Fawcett, J. W.; Jendelova, P.; Kwok, J. C. F.

doi:10.64898/2026.01.28.702127

bioRxiv · 10.64898/2026.01.28.702127

Chondroitin 4-sulphate depletion enhances synaptic plasticity and memory in aging

Svobodova Burianova, J.·Svoboda, J.·Ruzicka, J.·Stepankova, K.·Klausova, T.·Gmiterkova, L.·Spundova, T.·Martinez-Varea, N.·Kralikova, M.·Turecek, R.·Machova Urdzikova, L.·Fawcett, J. W.·Jendelova, P.·Kwok, J. C. F.

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Abstract

Perineuronal nets (PNNs), specialised extracellular matrix structures enriched in chondroitin sulphate proteoglycans (CSPGs), are key regulators of synaptic plasticity, learning, and memory. Aging is characterised by a shift in chondroitin sulphate composition toward increased chondroitin-4-sulfation (C4S) and reduced C6S, a pattern associated with declining cognitive flexibility. Here, we investigated how selective reduction of C4S affects PNN structure, PV-interneuron connectivity, and cognitive performance across the lifespan. Conditional deletion of the C4-sulfotransferase Chst11 markedly reduced C4S levels and diminished dendritic PNN complexity while preserving somatic PNN structure. This partial destabilisation of PNNs increased excitatory synaptic input onto PV interneurons in both young and aged mice, without major alterations in basal hippocampal transmission or long-term potentiation. Behaviourally, Chst11 knockout mice showed robust and persistent protection against age-related cognitive decline. Working memory performance remained stable across aging, short-term spatial memory was enhanced from early adulthood onward, and object recognition memory was significantly prolonged at all retention delays, even in old age. Sociability and social novelty preference were also preserved longer in aging knockouts compared with controls. These improvements occurred despite an overall preservation of PNN architecture, indicating that modifying sulphation rather than removing CSPGs is sufficient to enhance plasticity. Our findings demonstrate that reducing C4S through Chst11 deletion confers long-lasting enhancements in cognitive function and mitigates aging-related decline. Targeting CS-GAG sulphation patterns may therefore represent a promising strategy for maintaining cognitive resilience and restoring plasticity in aging or neurodegenerative conditions.

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Svobodova Burianova, J., Svoboda, J., Ruzicka, J., Stepankova, K., Klausova, T., Gmiterkova, L., Spundova, T., Martinez-Varea, N., Kralikova, M., Turecek, R., Machova Urdzikova, L., Fawcett, J. W., Jendelova, P., Kwok, J. C. F.. 2026-01-29. Chondroitin 4-sulphate depletion enhances synaptic plasticity and memory in aging. https://doi.org/10.64898/2026.01.28.702127

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