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bioRxiv · 10.64898/2026.01.14.699599

Isolation and Characterisation of Hair Follicle-Derived Melanocytes

Wu, F.·Liu, Q.·Shao, X.·Chen, S.·Lin, J.·Chen, J.·Li, J.·Zhang, Q.·Tan, Z. L.·Wu, W.
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Abstract

Melanocytes are unique population of cells which produce melanin pigment. However, the property of melanocytes from hair follicle and epidermis, particularly, their molecular signatures remained largely unknown. In this study, we investigated the properties of epidermis and hair follicle melanocytes by biochemical and molecular biological assays. We found that epidermal melanocytes are differentiated and mature form of hair follicle melanocytes, and they are potentially related. Irradiation with NB-UVB did not induce cell death of hair follicle melanocytes as observed in epidermal melanocytes, but activate hair follicle melanocytes, promote proliferation and migration. In situ study suggested that after UVB irradiation, root sheath of hair follicles, particularly proximity of interfollicular epidermis and hair bulb become darker, suggesting migration and maturation of hair follicle melanocytes after UVB irradiation, rather than cell death. This observation provides hints and evidence to the relationship between hair follicle and epidermal melanocytes, helps to explain the contradiction of melanocytes cell death after UVB irradiation and the use of UVB in treatment of vitiligo lesion, as well as the perifollicular repigmentation phenomenon observed in vitiligo lesion after NB-UVB phototherapy.

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Wu, F., Liu, Q., Shao, X., Chen, S., Lin, J., Chen, J., Li, J., Zhang, Q., Tan, Z. L., Wu, W.. 2026-01-15. Isolation and Characterisation of Hair Follicle-Derived Melanocytes. https://doi.org/10.64898/2026.01.14.699599

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