Pollutant biodegradation profile mediated by multi-trophic microbial dynamics in rivers

Abstract

Microbial communities and environmental conditions are closely linked to ecosystem functions and directly govern the biodegradation of pollutants in aquatic environments. However, the role of multi-trophic interactions and their spatiotemporal dynamics in these processes remains poorly understood. Here, we examined how seasonal and spatial variations, mediated by trophic interactions within benthic microbial communities, influence their composition, functional capacity, and collective potential to degrade a diverse array of organic pollutants in rivers. By characterizing both prokaryotic (i.e., archaea and bacteria) and eukaryotic taxa (i.e., algae, fungi, protists, and metazoans), and inferring metabolic pathways, we explored the connections between community composition and pollutant degradation in wastewater-receiving rivers across four seasons. Mediation analysis revealed that multi-trophic communities mediate the total effect of environmental factors on the biodegradation of 96 organic pollutants. Prokaryotic communities explained 60% of the total environmental influence on pollutant biodegradation. Additionally, eukaryotic groups had significant indirect mediation effects, with fungal, protistan, algal, and metazoan communities responsible for 56%, 53%, 26%, and 38%, respectively. Notably, fungal and protist communities mediated approximately 83% and 73% of the environmental impacts on prokaryotic community composition, respectively. Across the two rivers studied, spatial variation (at the river and reach scales) explained more variance in community composition than seasonality over the sampled year. Our findings improve understanding of ecosystem resilience and support the development of predictive models and sustainable water management strategies in dynamic aquatic environments.