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bioRxiv · 10.64898/2025.12.27.696707

Metabolic Responses of Different Levels of Fitness

San-Millan, I.·Martinez, J.
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Abstract

The metabolic and physiological responses to exercise vary markedly across different levels of fitness and training status. While maximal oxygen uptake (VO2max) has historically been used as the primary indicator of cardiorespiratory fitness, increasing attention has been directed toward metabolic and bioenergetic responses to exercise, including blood lactate concentration and substrate utilization. In this study, we examined comprehensive physiological and metabolic responses during graded exercise testing in 204 male cyclists spanning four fitness categories: Tour de France professional cyclists, competitive cyclists, master cyclists, and recreational cyclists. Measurements included power output, VO2, blood lactate concentration, and rates of fat and carbohydrate oxidation derived from indirect calorimetry. Across all exercise intensities, performance and metabolic parameters followed a clear hierarchical pattern corresponding to competitive level. Tour de France cyclists demonstrated significantly greater power output, higher VO2max, lower blood lactate concentrations at matched workloads, higher fat oxidation rates, and delayed reliance on carbohydrate oxidation compared with less-trained groups. Strong inverse correlations were observed between blood lactate concentration and fat oxidation, while positive correlations were observed between blood lactate concentration and carbohydrate oxidation across all fitness levels.

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San-Millan, I., Martinez, J.. 2025-12-27. Metabolic Responses of Different Levels of Fitness. https://doi.org/10.64898/2025.12.27.696707

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