Gas5A, a putative glucanosyltransferase from Botrytis, functions as cell death inducing protein in plants

Abstract

The necrotrophic fungus Botrytis cinerea, releases numerous phytotoxic, cell death inducing proteins (CDIPs) during infection. The precise role of these proteins and their molecular function, however, is still unknown. Here, we report on the identification of a previously unknown CDIP, the glucanosyltransferase Gas5A. Functional characterization revealed that the C-terminal 60 aa of Gas5A are sufficient to induce cell death, independent from its putative enzymatic function. Gas5A localization and functional dependence on the receptor-associated kinase suppressor of BIR1-1 (SOBIR1) and the plant defense regulator ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) indicate recognition as a pathogen-associated molecular pattern (PAMP) at the plant plasma membrane, but it is toxic also when delivered inside plant cells. Generation of a CRISPR/Cas9-assisted Botrytis knockout strain did not indicate any impact of Gas5A on virulence. Taken together, Gas5A represents a novel PAMP-like CDIP with additional intracellular phytotoxic activity.