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bioRxiv · 10.64898/2025.12.17.695041

OpenStride: an inexpensive, open-source force plate actometry system for quantification of rodent motor activity and behaviour

Yang, Y.·Cooper, B.·Houghton, M.·Dahiya, K.·Haslam, S. E.·Blackwell, H.·Sekaran, K.·Hu, L.·Kavehei, O.·Anderson, C. J.
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Abstract

IntroductionForce plate actometry (FPA) enables quantitative assessment of rodent motor and behavioural activity. Through tracking centre of mass at high temporal and spatial resolution, FPA can directly quantifying motor performance and various behaviours in the context of operator-independent, naturalistic movement. Previously designed systems have been expensive and non-customisable, and they are no longer commercially available. MethodsWe designed OpenStride, an open-source FPA system consisting of both hardware and software. Our system was designed to be able to be constructed with standard tools worldwide at a cost of [~]$550 USD, with modifiable hardware files, modular software, and support for both Mac and Windows operating systems. Required tools include 3D printing and acrylic laser cutting. ResultsOpenStride reliably tracks position within a 30 x 30 cm2 environment. Based on these positional data, OpenStride currently quantifies tremor, ataxia, distance, velocity, low-mobility bouts, and centre-vs-margin time, with potential to expand to additional analyses. ConclusionsOpenStride is intended to provide a valuable tool for high-throughput, inexpensive study of motor and behavioural function and dysfunction. Software and hardware files will be freely disseminated online via GitHub at the time of final publication to enable others to construct and utilise the OpenStride system.

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Yang, Y., Cooper, B., Houghton, M., Dahiya, K., Haslam, S. E., Blackwell, H., Sekaran, K., Hu, L., Kavehei, O., Anderson, C. J.. 2025-12-20. OpenStride: an inexpensive, open-source force plate actometry system for quantification of rodent motor activity and behaviour. https://doi.org/10.64898/2025.12.17.695041

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