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bioRxiv · 10.64898/2025.12.11.693653

Repurposed macroH2A Chromatin Landscapes Guide Cellular Reprogramming

Valakos, D.·Kokkalis, A.·Klagkou, E.·Polyzos, A.·Vatsellas, G.·Thanos, D.
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Abstract

Cellular reprogramming converts differentiated cells into a pluripotent state through extensive chromatin remodeling. The histone variant macroH2A has classically viewed as an epigenetic barrier stabilizing somatic identity and restricting pluripotency gene activation. Here, we show that during reprogramming, macroH2A1 nucleosomes undergo functional repurposing. Early in the process, mH2A1 nucleosomes act as a barrier to cellular plasticity, but later facilitate the establishment and maintenance of pluripotency by reshaping the epigenetic landscape. High-resolution chromatin profiling reveals that mH2A1.2 nucleosomes undergo rapid, large-scale repositioning, dissociating from promoters and reassembling [~]30 bp away, frequently near NRF-1 binding sites. This repositioning occludes E2F4 binding, relieving cell-cycle arrest thereby enabling reprogramming. Likewise, mH2A1.1 nucleosomes display extensive mobility that culminates in deposition at pluripotency genes in ESCs, where they sustain their expression by assembling promoter transcriptional hubs. In this later role, mH2A1.1 functions as a chromatin bookmark stabilizing the Nanog, Sox2, and Oct4 network. These findings suggest that mH2A1 nucleosomal mobility underlines its context-dependent functional repurposing from reprogramming inhibitor to facilitator, illustrating how chromatin components evolve dynamic roles to coordinate cell-state transitions.

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Valakos, D., Kokkalis, A., Klagkou, E., Polyzos, A., Vatsellas, G., Thanos, D.. 2025-12-12. Repurposed macroH2A Chromatin Landscapes Guide Cellular Reprogramming. https://doi.org/10.64898/2025.12.11.693653

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