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bioRxiv · 10.64898/2025.12.09.693215

Innovative Prevention Approach for Pan Allergens-Induced Allergy: Effective Regulation of Heat Shock Protein 70 and Profilin 3 by siRNA-Loaded Nanomaterial

Zheng, S. H.·Liang, Z.·Ren, B.·Yan, K.·Zhang, P.
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Abstract

Currently, the widely accepted approach to addressing pollen allergy primarily through altering the immune system of patients. However, the great potential of genetic engineering for downregulating allergenic proteins and reducing allergenicity is often overlooked in previous studies. Here, we report an effective solution to reducing plant allergenicity by utilizing RNA interference (RNAi) technology to calibrate levels of pan-allergenic proteins Heat Shock Protein 70 (HSP 70) and Profilin 3 (PFN 3) in transgenic Arabidopsis, Tobacco, and Osmanthus. This has been shown in research to effectively mitigate and prevent allergenic reactions in humans. 21-bp long siRNA sequences encased by Polyethyleneimine modified carbon dots (PEI-CDs) are synthesized to constitute a nanoparticle-mediated delivery system to efficiently deliver small-interfering RNA (siRNA) vectors into plants. Afterwards, RNA was extracted from Osmanthus fragrans and Gardenia jasminoides and underwent reverse transcription quantitative-polymerase chain reaction (RT-qPCR). The result indicated the expected suppression of mRNA corresponding to the targeted proteins, proving the functional aspect of RNAi in allergen suppression trunk injection and suggesting its applicability in real-life scenarios.

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Zheng, S. H., Liang, Z., Ren, B., Yan, K., Zhang, P.. 2025-12-10. Innovative Prevention Approach for Pan Allergens-Induced Allergy: Effective Regulation of Heat Shock Protein 70 and Profilin 3 by siRNA-Loaded Nanomaterial. https://doi.org/10.64898/2025.12.09.693215

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