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bioRxiv · 10.64898/2025.12.02.691861

Shrub and Sedge Rhizosphere Communities Display Distinct Affinities Toward Exudates and Soil Organic Matter Degradation: a Quantitative Stable Isotope Probing Analysis

Abstract

Warming temperatures are accelerating permafrost thaw and changing tundra vegetation, where woody shrubs are displacing sedges. Shrubs, such as Betula nana, and sedges, such as Eriophorum vaginatum, exhibit distinct life strategies including unique root-associated, or rhizosphere microbial communities. As permafrost thaws it unlocks previously unavailable carbon and nutrient sources resulting in deeper roots and a translocation of rhizosphere communities. Because permafrost microbial communities contain lower diversity and biomass than rhizosphere communities, the coalescence of rhizosphere and permafrost microbial communities could alter soil organic matter (SOM) degradation rates and increase greenhouse gas emissions. To identify metabolic strategies across distinct rhizosphere and permafrost microbial communities we conducted an isotope tracing incubation experiment. We inoculated thawed permafrost with shrub and sedge rhizosphere communities while adding exudates or water daily and compared this to an uninoculated control. After 46 days, we spiked samples with 18O enriched water or 13C enriched exudates and measured isotope incorporation into microbial DNA with quantitative stable isotope probing (qSIP). Our results indicate that exudate additions had little effect on uninoculated permafrost communities but the addition of exudates and rhizosphere inoculants had a compounding effect on respiration rates. We found that soils inoculated with shrub rhizosphere communities contained a mixture of exudate and SOM degraders while soils inoculated with sedge rhizosphere communities contained mainly SOM degraders. Finally, we found that individual microbial taxa exhibited maximum growth rates in one environment, which was a combination of microbial inoculant communities and exudate addition treatments. Our results reveal that microbial niches are strongly influenced by substrate preferences and community context, and suggest that a reduction in sedges and an expansion of shrubs may provide a mechanism by which permafrost carbon losses are mitigated through corresponding shifts in microbial communities and their substrate preferences.

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BibTeXRIS

Schaefer, S. R., Blazewicz, S. J., Holland-Moritz, H., Hicks Pries, C., Allen, G. M., Pett-Ridge, J., Lopez, F. M., Grandy, A. S., Wieder, W., Ernakovich, J.. 2025-12-02. Shrub and Sedge Rhizosphere Communities Display Distinct Affinities Toward Exudates and Soil Organic Matter Degradation: a Quantitative Stable Isotope Probing Analysis. https://doi.org/10.64898/2025.12.02.691861

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