The smfBox: an open-source platform for single-molecule FRET

Ambrose, B.; Baxter, J.; Cully, J.; Willmott, M.; Steele, E.; Bateman, B. C.; Martin-Fernandez, M. L.; Cadby, A.; Shewring, J.; Aaldering, M.; Craggs, T. D.

doi:10.1101/861922

bioRxiv · 10.1101/861922

The smfBox: an open-source platform for single-molecule FRET

Ambrose, B.·Baxter, J.·Cully, J.·Willmott, M.·Steele, E.·Bateman, B. C.·Martin-Fernandez, M. L.·Cadby, A.·Shewring, J.·Aaldering, M.·Craggs, T. D.

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Abstract

Single-molecule Forster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRETs broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.

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Ambrose, B., Baxter, J., Cully, J., Willmott, M., Steele, E., Bateman, B. C., Martin-Fernandez, M. L., Cadby, A., Shewring, J., Aaldering, M., Craggs, T. D.. 2019-12-05. The smfBox: an open-source platform for single-molecule FRET. https://doi.org/10.1101/861922

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