Cryo-OrbiSIMS for 3D molecular imaging of a bacterial biofilm in its native state

Zhang, J.; Brown, J.; Scurr, D.; Bullen, A.; MacLellan-Gibson, K.; Williams, P.; Alexander, M. R.; Hardie, K. R.; Gilmore, I. S.; Rakowska, P.

doi:10.1101/859967

bioRxiv · 10.1101/859967

Cryo-OrbiSIMS for 3D molecular imaging of a bacterial biofilm in its native state

Zhang, J.·Brown, J.·Scurr, D.·Bullen, A.·MacLellan-Gibson, K.·Williams, P.·Alexander, M. R.·Hardie, K. R.·Gilmore, I. S.·Rakowska, P.

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Abstract

We describe a method for label-free molecular imaging of biological materials, preserved in a native state, by using an OrbiSIMS instrument equipped with cryogenic sample handling and developing a high-pressure freezing protocol compatible with mass spectrometry. We studied the 3D distribution of quorum sensing signalling molecules, nucleobases and bacterial membrane molecules, in a mature Pseudomonas aeruginosa biofilm, with high spatial-resolution and high mass-resolution.

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Zhang, J., Brown, J., Scurr, D., Bullen, A., MacLellan-Gibson, K., Williams, P., Alexander, M. R., Hardie, K. R., Gilmore, I. S., Rakowska, P.. 2019-11-30. Cryo-OrbiSIMS for 3D molecular imaging of a bacterial biofilm in its native state. https://doi.org/10.1101/859967

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