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bioRxiv · 10.1101/859645

Primary mammary organoid model of lactation and involution

Sumbal, J.·Chiche, A.·Charifou, E.·Koledova, Z.·LI, H.
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Abstract

Mammary gland development occurs mainly after birth and is composed of three successive stages: puberty, pregnancy and lactation, and involution. These developmental stages are associated with major tissue remodeling, including extensive changes in mammary epithelium as well as surrounding stroma. Three-dimensional (3D) mammary organoid culture has become an important tool in mammary gland biology and enabled invaluable discoveries on pubertal mammary branching morphogenesis and breast cancer. However, a suitable 3D organoid model recapitulating key aspects of lactation and involution has been missing. Here, we describe a robust and straightforward mouse mammary organoid system modeling lactation and involution-like process, which can be applied to study mechanisms of physiological mammary gland lactation and involution as well as pregnancy-associated breast cancer.

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Sumbal, J., Chiche, A., Charifou, E., Koledova, Z., LI, H.. 2019-11-29. Primary mammary organoid model of lactation and involution. https://doi.org/10.1101/859645

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