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bioRxiv · 10.1101/854190

CHROMATIX: computing the functional landscape of many-body chromatin interactions in transcriptionally active loci

Perez-Rathke, A.·Sun, Q.·Wang, B.·Boeva, V.·Shao, Z.·Liang, J.
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Abstract

Chromatin interactions are important for gene regulation and cellular specialization. Emerging evidence suggests many-body spatial interactions can play important roles in condensing super-enhancer regions into a cohesive transcriptional apparatus. Chromosome conformation studies using Hi-C are limited to pairwise, population-averaged interactions; therefore, not suitable for direct assessment of many-body interactions. We describe a computational model, CHROMATIX, that reconstructs structural ensembles based on Hi-C data and identifies significant many-body interactions. For a diverse set of highly-active transcriptional loci with at least 2 super-enhancers, we detail the many-body functional landscape and show DNase-accessibility, POLR2A binding, and decreased H3K27me3 are predictive of interaction-enriched regions.

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Perez-Rathke, A., Sun, Q., Wang, B., Boeva, V., Shao, Z., Liang, J.. 2019-11-25. CHROMATIX: computing the functional landscape of many-body chromatin interactions in transcriptionally active loci. https://doi.org/10.1101/854190

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