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bioRxiv · 10.1101/852822

Visualizing a protonated RNA state that modulates microRNA-21 maturation

Baisden, J. T.·Boyer, J. A.·Zhao, B.·Zhang, Q.
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Abstract

MicroRNAs are evolutionarily conserved small, non-coding RNAs that regulate diverse biological processes. Due to their essential regulatory roles, microRNA biogenesis is tightly regulated, where protein factors are often found to interact with specific primary and precursor microRNAs for regulation. Here, using NMR relaxation dispersion spectroscopy and mutagenesis, we reveal that the precursor of oncogenic microRNA-21 exists as a pH-dependent ensemble that spontaneously reshuffles the secondary structure of the entire apical stem-loop region, including the Dicer cleavage site. We show that the alternative excited conformation transiently sequesters the bulged adenine into a non-canonical protonated A+-G mismatch, conferring a two-fold enhancement in Dicer processing over its ground conformational state. These results indicate that microRNA maturation efficiency may be encoded in the intrinsic dynamic ensemble of primary and precursor microRNAs, providing potential means of regulating microRNA biogenesis in response to environmental and cellular stimuli.

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Baisden, J. T., Boyer, J. A., Zhao, B., Zhang, Q.. 2019-11-23. Visualizing a protonated RNA state that modulates microRNA-21 maturation. https://doi.org/10.1101/852822

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