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bioRxiv · 10.1101/847665

Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Undergo Clonal Expansion In Vivo

El-Nachef, D.·Bugg, D.·Beussman, K. M.·Martinson, A. M.·Murry, C. E.·Sniadecki, N. J.·Davis, J.
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Abstract

Preclinical studies have suggested that transplanted human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) grafts expand due to proliferation. This knowledge came from cell cycle activity measurements that cannot discriminate between cytokinesis or DNA synthesis associated with hypertrophy. To refine our understanding of hPSC-CM cell therapy, we genetically engineered a cardiomyocyte-specific fluorescent barcoding system into an hPSC line. Since cellular progeny have the same color as parental hPSC-CMs, we could identify subsets of engrafted hPSC-CMs that clonally expanded, with the remainder being non-proliferative and hypertrophic.

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El-Nachef, D., Bugg, D., Beussman, K. M., Martinson, A. M., Murry, C. E., Sniadecki, N. J., Davis, J.. 2019-11-20. Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Undergo Clonal Expansion In Vivo. https://doi.org/10.1101/847665

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