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bioRxiv · 10.1101/839290

Challenges in Peptide-Spectrum Matching: a Robust and Reproducible Statistical Framework for Removing Low-Accuracy, High-Scoring Hits

Hubler, S. L.·Kumar, P.·Mehta, S.·Easterly, C.·Johnson, J.·Jagtap, P. D.·Griffin, T. J.
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Abstract

Workflows for large-scale (MS)-based shotgun proteomics can potentially lead to costly errors in the form of incorrect peptide spectrum matches (PSMs). To improve robustness of these workflows, we have investigated the use of the precursor mass discrepancy (PMD) to detect and filter potentially false PSMs that have, nonetheless, a high confidence score. We identified and addressed three cases of unexpected bias in PMD results: time of acquisition within a LC-MS run, decoy PSMs, and length of peptide. We created a post-analysis Bayesian confidence measure based on score and PMD, called PMD-FDR. We tested PMD-FDR on four datasets across three types of MS-based proteomics projects: standard (single organism; reference database), proteogenomics (single organism; customized genomic-based database plus reference), and metaproteomics (microorganism community; customized conglomerate database). On a ground truth dataset and other representative data, PMD-FDR was able to detect 60-80% of likely incorrect PSMs (false-hits) while losing only 5% of correct PSMs (true-hits). PMD-FDR can also be used to evaluate data quality for results generated within different experimental PSM-generating workflows, assisting in method development. Going forward, PMD-FDR should provide detection of high-scoring but likely false-hits, aiding applications which rely heavily on accurate PSMs, such as proteogenomics and metaproteomics.

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Hubler, S. L., Kumar, P., Mehta, S., Easterly, C., Johnson, J., Jagtap, P. D., Griffin, T. J.. 2019-11-12. Challenges in Peptide-Spectrum Matching: a Robust and Reproducible Statistical Framework for Removing Low-Accuracy, High-Scoring Hits. https://doi.org/10.1101/839290

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