Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy

Rieger, B.; Stallinga, S.; Heydarian, H.; Schueder, F.; Jungmann, R.; Ries, J.; Przybylski, A.; Bates, M.; Keller-Findeisen, J.; van Werkhoven, B.

doi:10.1101/837575

bioRxiv · 10.1101/837575

Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy

Rieger, B.·Stallinga, S.·Heydarian, H.·Schueder, F.·Jungmann, R.·Ries, J.·Przybylski, A.·Bates, M.·Keller-Findeisen, J.·van Werkhoven, B.

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Abstract

We present an approach for 3D particle fusion in localization microscopy which dramatically increases signal-to-noise ratio and resolution in single particle analysis. Our method does not require a structural template, and properly handles anisotropic localization uncertainties. We demonstrate 3D particle reconstructions of the Nup107 subcomplex of the nuclear pore complex (NPC), cross-validated using multiple localization microscopy techniques, as well as two-color 3D reconstructions of the NPC, and reconstructions of DNA-origami tetrahedrons.

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Rieger, B., Stallinga, S., Heydarian, H., Schueder, F., Jungmann, R., Ries, J., Przybylski, A., Bates, M., Keller-Findeisen, J., van Werkhoven, B.. 2019-11-10. Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy. https://doi.org/10.1101/837575

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