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bioRxiv · 10.1101/834663

Characterization of a Human Liver NIST Reference Material fit for Proteomics Applications

Davis, W. C.·Kilpatrick, L. E.·Ellisor, D. L.·Neely, B. A.
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Abstract

The National Institute of Standards and Technology (NIST) is creating new, economical, qualitative reference materials and data for proteomics comparisons, benchmarking and harmonization. Here we describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics, a reference material being developed. Consensus identifications using multiple search engines and sample preparations demonstrate a homogeneous and fit-for-purpose material that can be incorporated into automated or manual sample preparation workflows, with the resulting data used to directly assess complete sample-to-data workflows and provide harmonization and benchmarking between laboratories and techniques. Data are available via PRIDE with identifier PXD013608.

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Davis, W. C., Kilpatrick, L. E., Ellisor, D. L., Neely, B. A.. 2019-11-08. Characterization of a Human Liver NIST Reference Material fit for Proteomics Applications. https://doi.org/10.1101/834663

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